Search results for "molecular markers"
showing 10 items of 30 documents
Molecular markers and endometrial cancer
2022
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Crocus sativus L. Ecotypes from Mediterranean Countries: Phenological, Morpho-Productive, Qualitative and Genetic Traits
2021
The characterization of C. sativus ecotypes is of great interest for preserving them from a possible genetic erosion due to the decrease of European cultivation surface. In this study, we evaluated four ecotypes from Italy (Sardinia and Abruzzo), Spain (Castilla-La Mancha), and Greece (Kozani) in order to detect the existence of variability and promote the biodiversity of this crop. Thirty-one traits related to saffron flowering, flower morphology, production of spice and daughter corms, vegetative development (leaf and corm traits), and spice quality, were evaluated. In addition, a genetic analysis through three PCR-based approaches, SSRs, RAPD, and SRAP was assessed. Results highlighted a…
New polymorphic markers for genetic diversity studies in an invasive plant: the common ragweed (Ambrosia artemisiifolia L.)
2014
Common ragweed is an annual herb that belongs to the Asteraceae family. It is known as an invasive plant originating from the USA. In France the common ragweed is especially abundant in the Rhône-Alpes basin and currently spreads northwards in Burgundy. Ragweed colonizes different types of environments, such as railways, river sides, wastelands, farmlands and cultivated crops (especially sunflowers crops). Herbicide resistance to linuron, glyphosate and/or acetolactate synthase inhibitors has been reported in the USA, which complicates the chemical control of ragweed. In addition, the highly allergenic pollen of ragweed causes severe allergies. These elements make this plant a major threat …
The TP53 colorectal cancer international collaborative study on the prognostic and predictive significance of p53 mutation: influence of tumor site, …
2005
Purpose The aims of the TP53 Colorectal Cancer (CRC) International Collaborative Study were to evaluate the possible associations between specific TP53 mutations and tumor site, and to evaluate the prognostic and predictive significance of these mutations in different site, stage, and treatment subgroups. Patients and Methods A total of 3,583 CRC patients from 25 different research groups in 17 countries were recruited to the study. Patients were divided into three groups according to site of the primary tumor. TP53 mutational analyses spanned exons 4 to 8. Results TP53 mutations were found in 34% of the proximal colon tumors and in 45% of the distal colon and rectal tumors. They were assoc…
A contribution to the phylogeny and biogeography of the genus Isoetes (Isoetaceae, Lycopodiidae) in the Mediterranean region
2019
To better understand the phylogeny of Isoetes species in the Mediterranean region, sequences of the second intron of a LFY-like homolog were determined for six populations of three Mediterranean Isoetes species and analyzed with other LFY and ITS sequences of Euro-Mediterranean species available in GenBank. Nomenclature of selected species was updated according to recent literature. Investigations of voucher specimens from previously sequenced taxa revealed that a specimen of I. histrix from Crete, Greece used in several previous molecular studies is probably I. phrygia. Analysis of the limited LFY and ITS DNA sequence data presented here agrees with the organization of Mediterranean specie…
Somatic embryogenesis through in vitro anther culture ofCitrus sinensisL. Osbeck ‘Moro’
2019
In many crops, anther culture is the most used method to induce gametic embryogenesis, aimed to regenerate homozygous plants. However, also somatic embryogenesis can be obtained by this method, when somatic tissue is involved in regeneration process. Many factors can affect this procedure, such as genotype, pre-treatments applied to floral buds, pollen developmental stage, donor plant states, culture media composition and growth culture conditions. Anthers of Citrus sinensis L. Osbeck, cv. Moro, were collected at the vacuolate stage, and after a chilling (4°C) pre-treatment of 7 days, were placed on the same medium, evaluating different temperature stresses applied after the placing in cult…
Permanent genetic resources added to molecular ecology resources database 1 April 2010 - 31 May 2010
2010
Correspondance: Molecular Ecology Resources Primer Development Consortium, E-mail: editorial.office@molecol.com; International audience; This article documents the addition of 396 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Anthocidaris crassispina, Aphis glycines, Argyrosomus regius, Astrocaryum sciophilum, Dasypus novemcinctus, Delomys sublineatus, Dermatemys mawii, Fundulus heteroclitus,Homalaspis plana, Jumellea rossii, Khaya senegalensis, Mugil cephalus, Neoceratitis cyanescens, Phalacrocorax aristotelis, Phytophthora infestans, Piper cordulatum, Pterocarpus indicus, Rana dalmatina, Rosa pulverulenta, Saxifraga …
In vitro propagation of the relict laperinne’s olive (Olea europaea L. subsp. laperrinei)
2017
Olea europaea L. subsp. laperrinei (Oleaceae) is an endemic taxon of the mountainous regions of central Sahara, consisting of currently fragmented and small relict populations. The tree can propagate vegetatively or by seed, but no recent natural regeneration was observed in the Algerian massive populations, some of which are considered threatened with extinction. Sterile triploid individuals were also identified in some populations showing increasing vigour. As a result of its long persistence and despite its rarity, the Laperrine's olive is an iconic component of Saharan mountain ecosystems. The aim of this study is to develop an efficient micropropagation protocol for both diploid and tr…
Morphological, chemical, and genetic diversity of wild myrtle(Myrtus communis L.) populations in Sicily
2016
Myrtus communis L. İs a shrub widespread in the Mediterranean area. The interest in this species is growing, mainly due to its pharmacological and aromatic properties. The overexploitation of wild populations induced increasing degradation of plant cover with serious risk of loss of genetic diversity. This research explored the morphological, chemical, and genetic diversity of wild myrtle populations in Sicily, with the aim to provide a first characterization of a core collection of 36 accessions from 7 localities for future domestication programs. Amplified fragment length polymorphism fingerprinting generated 152 polymorphic fragments. STRUCTURE analysis identified three genetic clusters …
Evaluation of genetic variability and relatedness among eight Centaurea species through CAAT-box derived polymorphism (CBDP) and start codon targeted…
2021
Centaurea is a value-ultimate genus of medicinal plants showing high diversification levels, especially within the Mediterranean basin, and is still traditionally recognized as a complicated taxon. So far, few studies utilizing molecular markers have been done on Centaurea spp. towards a better dissection of its phylogeny and accurate assessment of genetic diversity. Here, two functional marker systems, start codon targeted (SCoT) polymorphism and CAAT box-derived polymorphism (CBDP), were implemented to assess the genetic diversity between eight wild Centaurea species in Egypt. Seventeen SCoT and 19 CBDP primers generated 197 and 179 bands, respectively. These primers generated 158 (80.2%)…